在低温下对甲化物对Pomacea canaliculate的甲杀虫作用机制研究
Huabao Chen1, Yue Zhou1, Min Zhang1
1College of Agronomy, Sichuan Agricultural University, Chengdu, China.
Pest management science
|March 8, 2024
概括
低温降低甲对果牛Pomacea canaliculata的有效性,因为它增加了免疫和压力基因的表达,特别是Cldf7和HSP70. 这种分子反应显著阻碍了软体动物杀虫.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 害虫管理 害虫管理 害虫管理
背景情况:
- 甲是控制侵入性果牛Pomacea canaliculata的关键软体杀虫剂.
- 水温显著影响甲的有效性,但潜在的分子机制尚不清楚.
研究的目的:
- 为了研究温度对甲的软体杀菌活性对P. canaliculata的影响.
- 阐明受低温影响的分子机制,特别是基因表达变化.
主要方法:
- 在不同温度下对甲的毒性进行系统的研究.
- 转录组分析以识别低温应激下基因表达变化.
- RNA干扰 (RNAi) 来评估特定基因 (Cldf7,HSP70) 在甲功效中的功能作用.
主要成果:
- 随着温度的降低,甲的杀菌活性显著下降 (LC50从25°C的0.82 mg/L增加到10°C的458.82 mg/L).
- 低温诱导了免疫,脂质合成和与氧化压力相关的基因的显著上调,包括MANF,HSP70,Cldf7,HSP60和PclaieFc.
- 通过RNAi介导的Cldf7和HSP70基因的淘汰导致P. canaliculata的死亡率分别增加了36.17%和48.90%,这表明它们在减轻甲毒性的作用.
结论:
- 低温通过上调P. canaliculata的Cldf7和HSP70基因表达来显著降低甲的疗效.
- 这些基因在牛的应激反应中起着至关重要的作用,影响金属的软体杀菌作用.
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