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沙门氏菌对酸盐的利用受到多个监管系统的抑制

Nicolas Wenner¹, Xiaojun Zhu¹, Will P M Rowe¹

¹Clinical Infection, Microbiology & Immunology, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.

|March 8, 2024

概括

沙门氏菌Typhimurium在酸盐上的生长受到新型RpoS独立系统的调节. 关键的调节者包括CspC,OxyS,IscR和RbsR,揭示了感染期间对碳代谢的复杂控制.

科学领域:

微生物学微生物学
细菌病原体的产生
分子生物学分子生物学

背景情况:

酸盐在肠道和巨细胞中起到免疫信号的作用,这些巨细胞是沙门氏菌Typhimurium的感染.
沙门氏菌Typhimurium表现出缓慢增长的酸盐,一个C4-二碳酸二氧化碳来源,与涉及西格玛因子RpoS.调节.
细菌中酸盐利用抑制的确切遗传机制尚未完全理解.

研究的目的:

在使用苏酸盐作为唯一的碳来源时,调查沙门氏菌Typhimurium增长抑制的遗传基础.
为了确定新的监管系统,超越RpoS,控制简要的利用.

主要方法:

采用实验性进化方法,用沙门氏菌Typhimurium生长在酸最小介质上.
隔离和分析快速生长的突变体,以揭示调节的遗传基础.
研究特定蛋白质和调节性RNAs在酸盐代谢中的作用.

主要成果:

识别了RpoS独立的系统,可以抑制酸盐利用.
发现的CspCRNA结合蛋白限制了糖酸的利用,由OxyS sRNA对抗.
发现IscR抑制了DctA传送器,而RbsR对于RpoS介导的抑制至关重要.

结论:

在沙门氏菌Typhimurium中酸盐的利用是由多个多余的监管系统控制的.
这些发现揭示了对中央碳代谢的调节及其在利基特定适应中的作用的新见解.
RpoS-依赖和RpoS-独立路径的相互作用提供了对酸盐代谢的严格控制.

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