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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 1, 2025

A Protocol for Real-time 3D Single Particle Tracking
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A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

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大FOV 3D定位显微镜通过空间变异的点扩散函数生成.

Dafei Xiao1, Reut Kedem Orange1, Nadav Opatovski1

  • 1Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa, Israel.

Science advances
|March 8, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了PPG3D,这是一种快速而准确的方法,用于在局部化显微镜 (LM) 中表征微观点扩散函数 (PSF). 这种技术可以在较大的视野 (FOV) 上改善超高分辨率成像.

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
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相关实验视频

Last Updated: Jul 1, 2025

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10:16

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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
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Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
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科学领域:

  • 光学显微镜是一种光学显微镜.
  • 超高分辨率成像成像技术
  • 生物物理学的生物物理.

背景情况:

  • 精确的点扩散函数 (PSF) 描述对于高性能局部化显微镜 (LM) 非常重要.
  • 传统的LM通常假定空间不变的PSF,这限制了大视野 (FOV) 成像中的性能.
  • 对空间变异的PSF进行核算对于先进的LM应用来说至关重要.

研究的目的:

  • 引入基于主要组件分析的准确和快速的现场依赖的3D PSF生成器和定位器 (PPG3D).
  • 为了使3D单分子定位显微镜 (SMLM) 在大型FOV上实现高准确度超分辨率成像.

主要方法:

  • 开发PPG3D,这是一种使用主要组件分析来生成PSF的新算法.
  • 通过模拟和实验3D SMLM成像进行验证.
  • 与现有的变速PSF发电机进行比较.

主要成果:

  • PPG3D在细胞结构 (线粒体,微管) 的超高分辨率成像中表现出有效性,在大型FOV上具有高保真度.
  • 与标准变速变量PSF发电机相比,精度提高了三倍.
  • 在基于图像平面的插值模式下,PPG3D的速度大约是现有的PSF生成器的100倍.

结论:

  • 在3D SMLM中,PPG3D为处理空间变异的PSF提供了准确和高效的解决方案.
  • PPG3D的用户友好性和速度表明它在SMLM和其他成像模式中具有广泛的适用性.
  • 这种方法增强了局部化显微镜用于大规模生物成像的能力.