主体代谢操纵基因通过整合性和结合性元素的快速传播
Elena Colombi1, Frederic Bertels2, Guilhem Doulcier3
1School of Agriculture, Food and Ecosystem Sciences, Faculty of Science, The University of Melbourne, Parkville, VIC 3010, Australia.
概括
在Pseudomonas syringae中,整合性和结合性元素 (ICE) 是多样化的,并携带辅助基因. 一个元素,Tn6212,操纵细菌的新陈代谢和基因表达,影响某些碳来源的健康.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 细菌进化的过程
背景情况:
- 整合性和结合性元素 (ICE) 是移动的遗传元素,可以转移基因,从而影响细菌的病原性和共生性等特征.
- 许多ICE携带了功能不明的基因,这代表了对它们在细菌进化和健康中的作用的理解存在重大差距.
- 最近ICE在流行病中获得的Pseudomonas syringae血统促使对这些元素的多样性和功能进行了调查.
研究的目的:
- 调查在Pseudomonas注射器物种复合体内的ICE的结构和功能多样性.
- 描述移动基因元素Tn6212在细菌健康和基因表达中的作用,这是PsICEs的常见载荷.
- 阐明Tn6212如何响应环境线索并操纵细菌新陈代谢.
主要方法:
- 生物信息分析用于识别和分类P. syringae物种复合体中的ICE.
- 基因操纵 (删除突变) 来评估Tn6212.2.的适应性影响.
- 用RNA测序 (RNA-seq) 来分析Tn6212对染色体基因表达的全球影响.
- 对各种碳来源进行生长测试,以评估代谢反应.
主要成果:
- 在P.syringae中发现了53种独特的ICE类型 (PsICEs),形成了一个独特的家族,具有保存的脊椎和可变的货物基因.
- 16kb移动基因元素Tn6212是最常见的货物,在删除后,导致三碳酸 (TCA) 循环中间体的适应性缺陷.
- 发现一个编码为Tn6212的LysR调节器会对染色体基因表达产生全球影响,并根据首选的碳来源对细菌代谢进行操纵.
结论:
- PsICEs 代表了 Pseudomonas 内的多样化和高度重组的移动元素家族.
- Tn6212在通过 LysR 调节器调节的细菌代谢适应特定营养环境方面发挥着至关重要的作用.
- 了解这些ICE编码的辅助基因对于理解细菌适应,进化和宿主-病原体相互作用至关重要.
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