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化抑制通过扰乱母细胞转化和小鼠线粒体功能来影响早期胚胎发育

Mingxiao Liu¹, Zhiming Ding², Peihao Sun¹

¹Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.

|March 8, 2024

概括

脱,一种关键的蛋白质修饰,对于小鼠早期胚胎发育至关重要. 抑制化阻断了胚胎发育,因为它破坏了基因表达,母体转化为胚胎的过渡,并导致氧化应激.

科学领域:

发育生物学发展生物学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

翻译后修改 (PTMs) 对于早期发育至关重要,因为在分裂阶段的胚胎中,转录不活跃.
化,一种类似于无处不在的PTM,调节重要的生物物理过程,但其在早期胚胎发生中的作用尚不清楚.

研究的目的:

调查化在调节小鼠早期胚胎发育中的作用.
阐明缩对胚胎进展影响的分子机制.

主要方法:

使用特异性抑制剂MLN4924抑制化.
在二细胞阶段进行转录组分析 (RNA-seq).
评估杂虫基因组激活,母体mRNA降解,线粒体功能,活性氧物种 (ROS) 水平和DNA损伤.

主要成果:

缩抑制导致了早期胚胎发育的严重停止.
转录组分析显示,在2细胞阶段,基因表达 (3959个基因) 发生了显著的变化.
尼迪化抑制阻断了胚胎基因组激活和母体mRNA降解,破坏了母体到胚胎的过渡.
观察到线粒体功能障碍,包括改变分布,减少膜潜力和减少ATP.
增加的ROS和超氧化离子积累导致氧化应激和DNA损伤.

结论:

在小鼠的早期胚胎发育中,化对成功的早期胚胎发育至关重要.
适当的化调节对于从母体过渡到胚胎过渡以及保持基因组完整性至关重要.
缩的破坏会导致通过线粒体功能障碍和氧化应激导致发育停止.

关键词:

胚胎发育的时间 MLN492424 没有使用从母体过渡到胚胎过渡的过程线粒体中的线粒体.尼迪化尼迪化尼迪化

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