一种多组件LC-MS/MS方法用于滥用药物测试,使用体积DBS和与尿液进行比较的临床评估
Joar Guterstam1, Christoffer Tavic2, Miguel Barosso2
1Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet & Stockholm Health Care Services, Stockholm County Council, Stockholm, Sweden.
Journal of pharmaceutical and biomedical analysis
|March 8, 2024
概括
针对尿液和干血斑点 (DBS) 开发了用于药物测试的新型多面板质谱法. 这些经过验证的方法为各种物质提供了敏感和准确的选,显示了在DBS中检测到更多阳性样本之间的良好一致性.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 传统的药物测试依赖于尿液免疫测试,需要监督收集.
- 质谱法 (MS) 能够进行广泛的多面板查,并使用其他样本,如干血斑 (DBS).
- 增加的药物物质需要超越传统方法的先进选方法.
研究的目的:
- 开发和验证使用液态染色体质谱法 (LC-MS) 的多面板选方法.
- 将这些方法适应尿液和DBS样本,用于药物滥用测试.
- 评估DBS在临床药物查中作为尿液替代品的实用性.
主要方法:
- 开发了一种使用液体/液体提取的37分析剂尿选方法.
- 开发了一种使用10μL体积DBS装置和两步溶剂提取的35分析DBS选方法.
- 采用稳定同位素标记的内部标准来量化这两种方法.
主要成果:
- 方法根据法医标准进行了验证,报告极限低 (100 ng/mL用于尿液,1 ng/mL用于DBS),准确度/不准确度可接受.
- 在所有样本中检测到甲;尿液结果有时低于标准切断值.
- DBS检测到的阳性病例比尿液更多,常见的共检测物质包括普雷加巴林,安非他胺和阿尔普拉佐拉姆.
结论:
- 成功开发了经过验证的多面板LC-MS尿液和DBS查方法.
- 这些方法适合临床药物查,提供了可行的替代样本与手指刺伤采集.
- 与尿相比,DBS分析在检测阳性病例方面显示出更高的灵敏度.
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