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Updated: Jul 1, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
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近期在活体中酶活性的比度光成像方面的进展

Emily D Cosco1, Matthew Bogyo2

  • 1Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

Current opinion in chemical biology
|March 8, 2024
PubMed
概括

电比度光学成像使用酶处理的光探针进行敏感,定量 in vivo 分子成像. 这种方法克服了不同疾病应用的剂量,照明和药理动力学的挑战.

关键词:
酵素探针探测器使用光探测器的光探测器光学成像技术的使用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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