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乌骨肌肉发育中的信号通路和调节网络:从全转录组测序的见解

Wentao Zhang¹, Jing Liu², Ya'nan Zhou¹

¹College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, P. R. China; Poultry Institute, Jiangxi Agricultural University, Nanchang 330045, P. R. China.

Poultry science

|March 8, 2024

概括

这项研究确定了长骨肌肉发育过程中的关键基因和非编码RNA变化. 调查结果显示,监管网络对于通过选择性繁殖优化家禽肉和蛋生产至关重要.

科学领域:

动物遗传学和发育生物学
禽畜科学禽畜科学禽畜科学
分子生物学分子生物学

背景情况:

是骨肌肉发育研究的有价值的鸟类模型,因为它们的尺寸和繁殖速度.
了解的骨肌肉发育对于改善家禽生产特征,如肉和蛋产量至关重要.
目前关于四骨肌肉发育的分子调节的知识有限.

研究的目的:

在骨肌发育过程中对基因和非编码RNA表达特征进行鉴定.
确定关键的调节途径和相互作用,涉及骨肌的分化在.
为改善家禽养殖计划提供基础.

主要方法:

组织学分析 (HE染色) 和定量PCR用于确定关键的发育时间点 (E9-E14).
整个转录组测序 (RNA-seq) 在 E9 和 E14 的腿肌肉上进行.
生物信息分析包括差异表达分析和调控网络构建 (lncRNA-mRNA,miRNA-mRNA等). ) 的情况.

主要成果:

甲的骨肌肉分化发生在胚胎第9天 (E9) 和胚胎第14天 (E14) 之间.
在E9和E14之间发现了大量差异表达的mRNA,lncRNA,circRNA和miRNA.
丰富分析揭示了不同的途径活动:细胞循环在E9 (增殖) 和ECM受体,焦点粘附,PPAR信号在E14 (新陈代谢,脂肪形成).
构建了涉及非编码RNA及其目标的监管网络.

结论:

这项研究提供了一个关于骨肌肉发育的全面的转录图形.
阐明了关键的非编码RNA及其在骨肌肉发育中的调节作用.
这些发现为家禽生产中的遗传改进策略提供了宝贵的见解.

关键词:

RNA监管网络的RNA监管网络是指RNA的监管网络.有差异表达的RNAs.甲甲甲甲的意思这是骨肌肉的骨架肌肉.整个转录基因组的测序.

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