在患有固体瘤的患者中,来自肠道微生物组的细菌细胞外囊泡
Surbhi Mishra1, Mysore Vishakantegowda Tejesvi2, Jenni Hekkala1
1Research Unit of Translational Medicine, University of Oulu, Oulu, Finland; Biocenter Oulu, University of Oulu, Oulu, Finland.
Journal of advanced research
|March 8, 2024
概括
来自固体瘤患者的细菌细胞外囊泡 (bEV) 显示出不同的蛋白质组,提供了比传统微生物组分析更准确的癌症检测方法. 这突出了bEVs作为潜在的诊断生物标志物.
科学领域:
- 微生物组研究的研究.
- 癌症生物学 癌症生物学
- 对纳米粒子进行分析.
背景情况:
- 细菌细胞外囊泡 (bEVs) 是来自肠道微生物群的纳米粒子.
- bEV含有蛋白质,mRNA,代谢物和脂质,提供了对宿主肠道微生物群相互作用的见解.
- 目前用于单独分析肠道微生物组合的方法可能无法完全捕捉宿主-肠道微生物组的通信,特别是在固体瘤患者中.
研究的目的:
- 为了比较固体瘤患者和健康对照的bEVs的蛋白质组和微生物组组成.
- 在固体瘤的背景下,研究bEVs在宿主肠道微生物群相互作用中的功能作用.
- 评估bEVs作为固体瘤诊断生物标志物的潜力.
主要方法:
- 从固体瘤患者的便样本和健康对照中分离bEV.
- 使用光谱学对bEV进行蛋白质组分析.
- 16S rRNA基因测序用于bEV和便样本的微生物群组成分析.
- 基于bEV和便微生物群数据进行样本分类的机器学习.
主要成果:
- 与对照组相比,固体瘤患者在bEV和便中表现出微生物群丰富性和多样性的减少.
- 来自患者的bEV蛋白质组更加多样化,并富含参与氨基酸/碳水化合物代谢和核酸结合的蛋白质.
- 机器学习分类使用便bEVs实现了100%的准确性,优于便样 (93%).
结论:
- bEVs代表了与整体肠道微生物群区别的独特功能实体.
- 与单独便微生物组分析相比,bEV显示出作为固体瘤更准确的诊断工具的希望.
- 除了传统的微生物组分析,对bEV的进一步研究对于癌症诊断和治疗至关重要.
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