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通过glsirt1介导的GlCAT脱甲基化调节了细胞内ROS水平,影响了Ganoderma lucidum中的甘酸生物合成

Jing Han¹, Lingshuai Wang¹, Xin Tang¹

¹Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.

Free radical biology & medicine

|March 8, 2024

概括

氨酸乙化影响了Ganoderma lucidum中的许多细胞过程,包括二次代谢物生物合成. 脱乙酶Glsirt1调节了酶活性和活性氧物种 (ROS) 水平,影响了甘酸的产生.

科学领域:

分子生物学分子生物学
生物化学生物化学
菌类学菌类学是指菌类学.

背景情况:

氨酸乙化是一种关键的,动态的蛋白质修饰. 它在Basidiomycetes中的非类蛋白质中的作用,如Ganoderma lucidum,并未得到充分理解.
脱乙酶GLSIRT1最近被确定为甘酸 (GA) 生物合成的调节者.

研究的目的:

调查G. lucidum中GLSIRT1-介导的氨酸乙化酶的特征,范围和生物作用.
通过比较野生类型的乙和Glsirt1沉默的G. lucidum的乙体来确定额外的Glsirt1基质.

主要方法:

蛋白质组分析比较野生类型的乙和Glsirt1-静止的Ganoderma lucidum突变的乙.
识别Glsirt1基质并分析它们在细胞通路中的参与.

主要成果:

广泛的GLsirt1-依赖的氨酸乙化影响了许多途径,包括能量代谢,蛋白质合成和G. lucidum的应激反应.
GlCAT被确定为直接的GLSIRT1标;其由GLSIRT1的脱乙基化降低了催化酶活性.
降低的催化酶活性导致活性氧物种 (ROS) 的增加,积极调节甘酸 (GA) 生物合成.

结论:

非希斯 lysine 乙化在 G. lucidum 的生物过程中起着重要作用.
通过GLsirt1调节ROS信号传递对于GA生物合成至关重要.
这项研究揭示了 lysine 乙化对细胞的广泛影响,以及 G. lucidum 中二次代谢产生的潜在干预点.

关键词:

乙基因组学是什么这种植物叫做Ganoderma lucidum.这就是GLCATAT的意义.格尔斯蒂尔特1是什么意思这就是ROSOS ROS.

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