黑鱼ATG16L1负面调节通过STING介导的抗病毒先天免疫反应
Yuqing Peng1, Xiaoyu Liu1, Shasha Tan1
1State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
Fish & shellfish immunology
|March 8, 2024
概括
与自相关的16像1 (ATG16L1) 负面调节了黑鱼中干扰素基因 (STING) 途径的刺激器. 这种相互作用抑制了STING的激活,减少了抗病毒反应,促进了病毒逃避.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 干扰素 (IFN) 生产对于抗病毒防御和宿主恒温至关重要.
- 干扰素基因刺激器 (STING) 是哺乳动物抗病毒信号传输中的关键适应器.
- 游泳池STING的监管机制仍未得到充分探索.
研究的目的:
- 调查黑鱼自相关的16像1 (bcATG16L1) 在bcSTING信号通路中的作用.
- 阐明bcATG16L1对bcSTING介导的抗病毒免疫的调节机制.
主要方法:
- 在黑鱼细胞中,bcATG16L1的敲除.
- 对IFN和ISG转录水平的分析.
- 对抗病毒活性的评估.
- 对bcSTING促进体激活的调查.
- 蛋白相互作用和亚细胞局部化研究.
- 对bcSTING寡合化的评估.
主要成果:
- 抑制bcATG16L1增加了IFN和ISG转录,并增强了抗病毒活性.
- bcATG16L1抑制了bcSTING介导的IFN促进器激活和抑制了抗病毒能力.
- bcATG16L1与bcSTING相互作用,显示了类似的亚细胞分布.
- bcATG16L1减弱了bcSTING的寡合化,这是STING激活的关键步骤.
结论:
- bcATG16L1作为黑鱼bcSTING信号通路的负调节器.
- bcATG16L1通过抑制STING寡合化来抑制bcSTING介导的抗病毒反应.
- 这项研究揭示了一个新的调节机制,用于 piscine STING-依赖免疫力.
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