相关实验视频

Updated: May 7, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

开发一种先进的液体染色学-双重质谱测量系统,用于同时进行脂分析

Baasanjav Uranbileg¹, Eri Sakai^1,2, Masayuki Kubota²

¹Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.

Scientific reports

|March 8, 2024

概括

一种新的液体染色体质谱法方法可以在各种生物样本中同时检测多个脂体 (Sph,S1P,Cer1P,SM). 这一进展有助于理解脂在疾病发病过程中的作用.

科学领域:

利皮多米克 (Lipidomics) 是一种消化剂.
质谱测量质量谱测量
生物化学生物化学

背景情况:

斯芬戈脂在细胞过程和疾病的发病过程中起着至关重要的作用.
准确和全面的定量各种类型的脂是研究的必要条件.
现有的方法可能缺乏在各种生物矩阵中同时检测的能力.

研究的目的:

开发和验证一种新的,单列液体染色体质谱法 (LC-MS) 方法.
为了能够同时检测和量化多种脂类物种.
将该方法应用于各种生物样本,包括细胞溶解物和血清.

主要方法:

使用了一个单列LC-MS系统,具有正常相分离和正电离.
在稀释的血清样本中验证了精度 (CV < 20%) 和线性方法.
测量到的斯芬戈辛1-酸盐 (S1P),斯芬戈辛 (Sph),胺1-酸盐 (Cer1P),斯芬戈米林 (SM) 和其他相关的脂.

主要成果:

开发的LC-MS系统显示了高精度和线性,用于脂量化.
成功测量了一组关键脂组,包括S1P,Sph,Cer1P和SM.
在细胞溶解物中观察到显著和动态的脊脂蛋白调节,具有改变的S1P溶解酶表达.

结论:

已经建立了一种验证的LC-MS方法,用于生物样本中的同时脂分析.
这种方法提供了一种全面的方法来检测脂变化.
该系统是实验室测试和推进研究脂介导病原体的有前途的工具.

关键词:

胺1-酸盐 (Cer1P) 是一种脱氧基胺的使用方法脱氧化-dH-胺黑基胺 (HexCer) 是一种乳糖胺 (LacCer) 是一种乳糖胺.斯芬戈脂质是一种脂质.这就是DH-陶胺.

更多相关视频

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...