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病毒基因组测序来破译在医院的SARS-CoV-2传播事件

Elisabeth Esser^1,2, Eva C Schulte^1,3,4,5,6, Alexander Graf⁷

¹Institute of Virology, School of Medicine & Health, Technical University of Munich/Helmholtz Munich, Munich, Germany.

Scientific reports

|March 8, 2024

概括

对SARS-CoV-2的全基因组测序确定了传统的接触追踪遗漏的隐藏的医院传播链. 这种方法可以加强医疗机构对传染病的监测.

科学领域:

传染病流行病学传染病流行病学
基因组监测基因组监测
在医院获得的感染.

背景情况:

随着COVID-19的爆发,人们越来越需要强大的方法来追踪医院内疾病的传播.
了解医院内感染对于患者安全和有效的医疗管理至关重要.

研究的目的:

评估整个病毒基因组测序在大学医院内识别SARS-CoV-2传播的实用性.
以基因组测序为基础的传染集群与传统采访为基础的接触追踪产生的传染集群进行比较.

主要方法:

从慕尼黑大学医院的患者和工作人员收集了926个SARS-CoV-2病毒基因组 (2020年2月 - 2021年1月).
分析病毒基因组以及临床数据 (工作场所,住院时间,病房转移).
将生物信息学推断的传染集群与接触追踪数据和外部医院数据进行比较.

主要成果:

对619个基因组的集群分析确定了19个传播集群 (3-31个个体).
基因组测序揭示了接触追踪无法检测到的传播事件.
来自研究医院的病毒基因组比来自其他医院的病毒基因组更密切,这表明病房传播.
纵向采样表明,在19.2%的患者中出现了交叉感染.

结论:

整个SARS-CoV-2基因组测序有效地识别了常规接触追踪错过的神秘传播事件.
基因组测序是医院环境中病原体传播监测的宝贵工具.
这种方法可以提高对医疗保健设施内的传染病传播的理解和控制.

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