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循环的miRNA与不同血清维生素D组的儿童喘住院病史有关

Xiaoning Hong¹, Mingye Jiang¹, Alvin T Kho^2,3

¹Clinical Big Data Research Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong, China.

Respiratory research

|March 8, 2024

概括

维生素D水平会影响与儿童喘恶化相关的微RNA (miRNA). 特定的miRNA可以预测恶化,突出显示它们在免疫路径和维生素D中的作用.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

维生素D的抗炎作用可能有助于喘,但对儿童的证据是混合的.
微RNAs (miRNAs) 是喘发展的关键,并作为非侵入性生物标志物.
这项研究调查了循环miRNAs,喘恶化和维生素D状态之间的联系.

研究的目的:

为了识别与儿童喘恶化相关的循环miRNAs.
为了确定维生素D水平是否会改变miRNAs和喘恶化之间的关联.
探索已识别的miRNAs在免疫路径中的功能性作用.

主要方法:

461名儿童喘管理计划 (CAMP) 参与者的血清miRNA测序.
对miRNA恶化相关性的逻辑回归分析,按维生素D充足度分层.
在体外验证使用用维生素D治疗的淋巴细胞B细胞;用于功能丰富的权重基因同表达网络分析 (WGCNA).

主要成果:

11个miRNA与喘恶化有关,具有显著的维生素D效应修饰.
在维生素D不足组中,有5个miRNA显著,在足够组中有9个miRNA显著.
六个miRNA与免疫相关的基因模块相关,这表明维生素D通过免疫路径对其作用的调解. 确定了hsa-miR-143-3p和hsa-miR-451a作为潜在的预测因素.

结论:

循环中的miRNAs是儿童喘恶化的潜在媒介.
维生素D水平直接影响了miRNAs和喘恶化之间的关联.
特定的miRNA可以作为预测儿童喘恶化的生物标志物,由维生素D状态调节.

关键词:

喘喘是一种儿童喘在儿童喘.在循环中的miRNA.恶化的加剧.住院治疗住院治疗维生素D 维生素D是一种维生素.这是一个小RNARNA.

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