慢性HBV倾向的IL-18和CD14变体:一个病例控制研究,在体分析中,重点是转录和拼接
Mohammad Sarhadi1, Elham Pahlavani2, Niloufar Hosseini Razavi1
1Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran.
Nucleosides, nucleotides & nucleic acids
|March 9, 2024
概括
在IL-18和CD14的遗传变异影响慢性乙型肝炎病毒 (HBV) 感染的风险. IL-18-rs187238 C>G变异作为抗慢性HBV的保护因素,而其他组合基因型增加风险.
科学领域:
- 免疫遗传学 免疫遗传学
- 肝病学 肝病学是一种肝病学.
- 生物信息学是一种生物信息学.
背景情况:
- 乙型肝炎病毒 (HBV) 感染是一个全球性的健康问题,可能导致严重的肝脏疾病,如肝硬化和肝细胞癌.
- 免疫反应的个体差异,受细胞因子的遗传变异的影响,影响HBV的临床表现.
- 免疫遗传学分析有助于了解疾病的易感性和进展.
研究的目的:
- 研究IL-18 (rs187238 C>G,rs1946518 T>G) 的促进子区域和CD14 (rs2569190 A>G) 的内在区域的特定遗传变异与慢性HBV感染风险之间的关联.
- 用生物信息学工具分析这些遗传变异的功能影响.
主要方法:
- 使用聚合酶链反应-限制片段长度多态 (PCR-RFLP) 的400个个体 (200例,200对照) 的基因定型.
- 生物信息分析用于评估基因组保存,转录,拼接和蛋白质相互作用.
- 统计分析以确定基因型,单元型和慢性HBV风险之间的关联.
主要成果:
- 该IL-18-rs187238C>G变异显示了对慢性HBV的保护作用 (OR=0.62,95%CI:0.46-0.83,p=0.002).
- 特定的组合基因型 (TG/CC/AA,TG/CC/AG,TT/CC/AG,GG/CC/AA) 显著增加了慢性HBV风险 (p<0.05).
- IL-18 G/T和G/G单元类型与降低风险相关 (p<0.05). 生物信息学表明IL-18-rs187238 C>G和CD14-rs2569190 A>G变异的功能作用.
结论:
- IL-18-rs187238 C>G 多态性是预防慢性HBV感染的重要保护因素.
- 在IL-18和CD14的遗传变异在调节慢性HBV的风险方面发挥作用.
- 在更大,多样化的群体中进行进一步的研究是有必要的,以确认这些发现并探索潜在的机制.
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