神经肌肉电刺激对人类骨肌肉转录组的影响
Johanna Flodin1,2, Stefan M Reitzner3,4, Eric B Emanuelsson3
1Integrative Orthopedic Laboratory, Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
Acta physiologica (Oxford, England)
|March 9, 2024
概括
神经肌肉电刺激 (NMES) 子诱导骨肌肉的基因表达变化,类似于运动. 这表明NMES可以为那些无法进行传统炼的人提供健康和绩效益.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 生物医学工程 生物医学工程
背景情况:
- 与传统运动相比,神经肌肉电刺激 (NMES) 对骨肌肉的分子效应尚不清楚.
- 在研究NMES后四头肌肌的急性转录组变化对于理解其生理影响至关重要.
研究的目的:
- 为了比较单次NMES会话引起的四头肌肌肉的全球mRNA表达变化与自愿膝盖伸展运动 (EX) 相比.
- 评估与NMES相关的不适程度.
- 探索NMES的潜在健康和性能益处.
主要方法:
- 用RNA测序分析了30名健康参与者在30分钟的NMES或EX会话之前和之后的横向巨大的肌肉中的基因表达.
- 在20%的最大自发收缩 (MVC) 时使用集成的织电极应用NMES,并通过视觉模拟尺度 (VAS) 测量不适.
- 运动 (EX) 在80%的1重复-最大.
主要成果:
- 20%MVC的NMES诱导了4448个差异表达基因 (DEG),80%的重叠与EX后发现的2571个DEG.
- 关键的运动相关基因 (如PPARGC1A,VEGFA) 也被NMES上调.
- 无论是NMES还是EX都激活了共同的基因通路,尽管有些通路是每个模式的独家 (例如,NMES的神经元外生,EX的肌肉增殖).
结论:
- 在20%的MVC下使用NMES子进行30分钟的NMES会话,在骨肌中引起了显著的,类似运动的分子反应,并引起了可以接受的不适.
- NMES显示了诱导类似于运动的有益分子适应的潜力.
- 对于那些无法执行常规抗力运动的人来说,NMES可能是一个可行的替代方案,可能会导致健康和表现的改善.
相关概念视频
01:22Muscle Stimulation Frequency
2.2K
The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
2.2K
01:20Motor Unit Stimulation
1.6K
When the neuron of a motor unit fires an action potential, it triggers a series of events, leading to a twitch contraction in the muscle fibers. The process of excitation-contraction coupling is crucial in relaying the action potential to the muscle fibers.
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
The latent period of contraction marks the onset of excitation-contraction coupling, when the action potential propagates across the sarcolemma, preparing the muscle fibers for contraction. As the fibers enter the contraction phase, the...
1.6K