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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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在植物转化在小麦:一个改进的协议,以开发小麦转化剂.

Satish Kumar1, Harinder Vishwakarma1, Gourab Ghosh1

  • 1ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.

Molecular biology reports
|March 9, 2024
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概括

研究人员开发了一种更简单,更快速的小麦转化方法. 转基因植物的早期选 (T0) 减少了工作量而不损失效率,在小麦基因型HD2894.4中实现了3.33%的转化.

关键词:
美国GFP分析在工厂内进行转换.这是一个PCRPCR.射击顶端的美里系统.南方杂交的混合化小麦小麦小麦的小麦.

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科学领域:

  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学
  • 农业科学 农业科学

背景情况:

  • 有效的转换协议对于小麦基因组编辑和转基因开发至关重要.
  • 以前的印度面包小麦的植物加工方法是劳动密集型和耗时的.
  • 这项研究旨在改进植物内转化方法的简单性,效率和减少劳动力.

研究的目的:

  • 为小麦制定一个简化和高效的植物性转化协议.
  • 为了减少小麦转化所需的劳动和时间.
  • 建立一种产生转基因和基因组编辑小麦系的方法.

主要方法:

  • 小麦苗苗的注射射射出了带有GFP基因的Agrobacterium tumefaciens的角干.
  • 在T0阶段为转基因阳性植物引入基于PCR的选.
  • 使用PCR和光显微镜分析T1基因生成植物的稳定转基因整合.

主要成果:

  • 在T0阶段的PCR查发现了27种转基因阳性植物.
  • 在6种PCR阳性T1植物中,4种显示稳定的转基因整合 (GFP).
  • 光显微镜证实了南方4种混合阳性T1植物中的GFP表达.

结论:

  • T0阶段选有效地减少了下一代工厂的数量,而不会影响转化效率.
  • 一种简单,高效,劳动密集度较低的方法在小麦基因型HD2894.4.中实现了3.33%的转换效率.
  • 改进的方法有助于开发具有理想特征的转基因和基因组编辑小麦.