对唾液样本的不同提取方法和溶剂的比较
Lingli Fang1, Qiming Zhai1, Hua Zhang2
1Chongqing Key Laboratory of Oral Disease and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing, China.
在一般的唾液代谢物分析中,建议用酸或进行蛋白质沉 (PPT). 使用和甲醇的液体-液体提取 (LLE) 对于小型疏水化合物是最好的.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
背景情况:
- 唾液代谢产物为口腔,肠道和全身疾病提供了洞察力.
- 从唾液中有效地提取代谢物对于准确的分析至关重要.
- 现有的提取方法 (PPT,LLE,SPE) 在效率和溶剂使用方面各不相同,需要进行系统的评估.
研究的目的:
- 为了确定液代谢物提取的最佳方法和溶剂.
- 根据特定的分析目标来定制提取策略.
主要方法:
- 采用一种非向的代谢学方法,使用液态染色体质谱法 (LC-MS).
- 基于代谢物覆盖,数据重复性和信号强度的评估提取方法.
主要成果:
- 蛋白沉 (PPT) 显示了最高的代谢物覆盖率.
- 在PPT中,乙二提供了最佳的重复性和覆盖率.
- 在PPT中,乙产生了最高的信号强度.
- 使用/甲醇进行液液提取 (LLE),在提取小型疏水化合物方面表现出色.
结论:
- 建议在非目标唾液代谢中使用乙二或的PPT.
- 建议用甲/甲醇进行LLE,以有针对性地提取小型疏水性代谢物.
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