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在中预测功能性SNP中断抗氧化剂防御基因与COVID-19进展有关的功能性SNP

Somayeh Hashemi Sheikhshabani¹, Soudeh Ghafouri-Fard^1,2, Zeinab Amini-Farsani²

¹Student Research Committee, Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Biochemical genetics

|March 9, 2024

概括

抗氧化剂防御基因的遗传变异显著影响COVID-19的易感性和进展. 这些单核酸多态 (SNP) 影响基因功能和药物反应,解释了疾病严重程度和治疗结果的个体差异.

科学领域:

遗传学和分子生物学
计算生物学计算生物学
免疫学免疫学免疫学

背景情况:

反应性氧物种和抗氧化防御之间的不平衡在疾病发展中至关重要.
个体遗传变异导致疾病易感性差异,包括COVID-19等感染.

研究的目的:

通过计算预测抗氧化剂防御基因中单核酸多态 (SNPs) 对其结构,功能和 COVID-19 相关表达的影响.
研究SNP如何影响miRNA结合部位,影响药物反应和COVID-19易感性.

主要方法:

在关键抗氧化基因 (GPX7,GPX8,TXNRD2,GLRX5,GLRX,GPX,PRDX,GLRX,TXN,SOD) 中SNP的分析.
使用计算工具预测SNP对蛋白质结构,功能和miRNA结合部位的影响.
使用GEO2R.的轻度与重度COVID-19患者的基因表达特征的比较.

主要成果:

预计六个错误的SNP (MAF ≥0.1) 会显著改变抗氧化基因的结构和功能.
发现39个SNP (MAF ≥0.1) 在抗氧化剂基因中产生或取消miRNA结合位.
表达式分析显示,在轻度和严重的COVID-19病例之间,约250个miRNA发生了显著的变化,其中21个因SNP而改变了结合效率.

结论:

抗氧化剂防御基因中的SNP在COVID-19的进展和个体易感性方面发挥着根本性的作用.
对COVID-19抗氧化剂增强药物的患者反应观察到的变异可能归因于这些遗传变异的存在.

关键词:

抗氧化剂防御的抗氧化剂防御生物信息学是一种生物信息学.在 COVID-19 疫情中,在SNP中,SNP是SNP.微型RNA (miRNA) 是一种微型RNA.

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