在迁徙的原鹿 (Rangifer tarandus) 中检测和量化与数字皮肤炎相关的Treponema物种
Angelica P Dias1, Xavier F Aguilar2, Jeroen De Buck1
1Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z6, Canada.
Research in veterinary science
|March 9, 2024
概括
在健康的北极大猩猩中发现了与病相关的细菌特雷波涅玛物种. 这一发现扩大了我们对这些病原体在野生反动物中的理解,以及它们在的潜在作用.
科学领域:
- 兽医微生物学 兽医微生物学
- 野生动物疾病生态学 野生动物疾病生态学
背景情况:
- 特雷波内马物种与牲畜和野生反动物的传染性病有关.
- 病在北极大猩猩 (Rangifer tarandus) 中普遍存在,但研究有限.
- 牛数字性皮肤炎是牛的重大问题,是由Treponema引起的.
研究的目的:
- 调查野生反动物鹿中Treponema物种的存在和流行情况.
- 评估在鹿中发现的Treponema与家禽反动物中发现的Treponema的遗传相似性.
- 探索Treponema在鹿的潜在作用.
主要方法:
- 从48只大鹿的冠状动脉带组织中提取DNA,没有明显的损伤.
- 分析使用定量PCR (qPCR) 和嵌套PCR (nPCR) 来检测特雷波尼马.
- 用PCR产品的桑格测序来确认物种识别.
主要成果:
- 在鹿冠状动脉带组织中检测到Treponema物种.
- 最常见的物种是Treponema phagedenis (58%),其次是T. medium (44%) 和T. pedis (10%).
- 测序显示,鹿中的Treponema菌株与家禽的反动物和鹿的Treponema菌株相似.
结论:
- 特雷波内马殖民于健康的鹿,甚至在没有牲畜的偏远北极环境中.
- 这项研究扩大了Treponema物种已知的宿主范围和分布.
- 需要进一步研究特雷波尼马相关爪疾病在鹿的多因素性质.
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