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通过减弱的总反射率-里埃变换红外光谱学与第一阶衍生物处理来区分和量化Panax多糖提取物

Zhi-Ping Yan¹, Fang-Yu Zhou¹, Jun Liang¹

¹Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Harbin 150040, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|March 9, 2024

概括

这项研究引入了一种快速方法,使用减弱总反射率 - 里埃变换红外光谱学 (ATR-FTIR) 与衍生处理来区分和量化Panax人参多糖 (PGP),Panax notoginseng多糖 (PNP) 和Panax quinquefolius多糖 (PQP). 这种方法在分析这些重要的植物化合物方面被证明是有效和准确的.

科学领域:

分析化学分析化学
频谱学是一种光谱学.
化学测量化学测量化学测量

背景情况:

导数光谱学通过隔离小的吸收峰值来增强光谱分析,提高数据质量.
准确识别和定量Panax物种多糖是质量控制和研究的关键.
分析Panax多糖的现有方法可能耗时或破坏性.

研究的目的:

开发一种快速,非破坏性的方法,用于对Panax人参多糖 (PGP),Panax notoginseng多糖 (PNP) 和Panax quinquefolius多糖 (PQP) 的定性和定量分析.
为了利用减弱的总反射率 - 里埃变换红外光谱学 (ATR-FTIR) 结合一阶导数 (FD) 处理和化学计量用于此目的.
建立可靠的区分和定量模型,用于区分和测量Panax多糖含量.

主要方法:

减弱的总反射率-里埃变换红外光谱学 (ATR-FTIR) 与第一阶导数 (FD) 处理.
化学测量技术包括主要成分分析 (PCA),部分最小平方差分分析 (PLS-DA) 和线性差分分析 (LDA) 进行分类.
开发使用部分最小平方 (PLS) 的二维ATR-FTIR (2D-sATR-FTIR) 和定量回归模型.

主要成果:

开发的歧视模型成功地区分了PGP,PNP和PQP.
2D-sATR-FTIR方法提供了有效的三种Panax多糖的差异化.
优化的PLS回归模型显示了Panax多糖提取物含量的良好预测准确性.
综合方法提供了一种高效,经济和非破坏性的分析策略.

结论:

配合FD处理和化学测量的ATR-FTIR提供了一种高效准确的方法,用于快速区分和量化PGP,PNP和PQP.
该战略为涉及Panax物种的质量控制和研究提供了有价值的工具.
该方法的非破坏性使其适用于分析有价值或有限的样本.

关键词:

在ATR-FTIR中使用.化学测量化学测量化学测量第一个顺序的衍生谱法.帕纳克斯的多糖提取物.

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