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酸酶可降解的纳米粒子提供持续的药物释放

Simona Summonte¹, Eva Sanchez Armengol², Fabrizio Ricci¹

¹Department of Pharmaceutical Technology, University of Innsbruck, Institute of Pharmacy, Center for Chemistry and Biomedicine, 6020 Innsbruck, Austria; Thiomatrix Forschungs- und Beratungs GmbH, Trientlgasse 65, 6020 Innsbruck, Austria.

International journal of pharmaceutics

|March 9, 2024

概括

开发了带有乙基 (ETH) 的酶降解多酸盐纳米粒子 (NP). 和聚酸NP显示持续ETH释放由酸酶 (ALP) 触发,保持对大肠杆菌的抗菌活性.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
药物输送系统药物输送系统

背景情况:

开发新型药物输送系统对于提高治疗疗效至关重要.
酶反应性材料提供有针对性的药物释放机制.
聚酸纳米颗粒为药物封装提供了一个有前途的平台.

研究的目的:

设计可酶降解的聚酸纳米粒子 (NP),以持续释放乙基 (ETH).
研究双价金属 (,,铁) 对NP特性和药物释放的影响.
为了评估释放的ETH的抗菌疗效.

主要方法:

通过与ETH的共同沉合成,和铁聚酸盐NP.
对NP进行尺寸,PDI,泽塔潜力,封装效率和药物负载的表征.
使用血液溶解和细胞活力试验评估NP毒性.
评估性酸酶 (ALP) 的酶降解和随后的药物释放.
对大肠杆菌的抗菌活性的确定.

主要成果:

合成的NP范围在300-480nm之间,具有负泽塔电位和高封装效率 (83.73%-95.99%).
NP表现出良好的血红相容性,对HEK-293细胞活性的度依赖性影响.
和聚酸NP在ALP治疗后显示了泽塔潜力,颗粒大小和药物释放的显著变化.
从Ca-和Zn-PPNP中释放的ETH保留了对大肠杆菌的强烈抗菌活性.

结论:

聚酸纳米颗粒与双价离子交联,为持续的药物输送提供了一个可行的策略.
从这些纳米载体中释放ALP触发的药物有潜在的亲肠道给药.
开发的NP显示了针对性抗菌疗法的前景.

关键词:

具有抗菌活性,具有抗菌活性.由酶触发的纳米载体.金属化物是如何形成的酸酶是一种酸酶.聚酸纳米颗粒是一种多酸纳米颗粒.持续的药物释放持续的药物释放

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