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微RNA-18a通过准CTDSPL来阻止中细胞干细胞的衰老

Bo Sun¹, Xian-Hui Meng¹, Yu-Min Li¹

¹State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

|March 9, 2024

概括

在中细胞干细胞 (MSCs) 中,CTDSPL诱导过早衰老. 微RNA-18a-5p (miR-18a-5p) 防止这种衰老,在长期培养过程中保持MSC自我更新和功能.

科学领域:

干细胞生物学干细胞生物学
分子生物学分子生物学
细胞衰老细胞衰老

背景情况:

介质干细胞 (MSC) 对再生医学至关重要,但在长时间的体外扩张过程中经历衰老,限制了它们的治疗潜力.
了解驱动MSC衰老的分子机制对于改进细胞治疗方案至关重要.

研究的目的:

调查CTDSPL在MSCs过早衰老中的作用.
阐明miR-18a-5p在调节MSC衰老和自我更新中的作用.

主要方法:

在MSC中过度表达CTDSPL和miR-18a-5p.
对衰老标志物的评估 (形态,p16,SA-β-gal,细胞周期).
路西法酶记者测定证实了CTDSPL的miR-18a-5p调节.
评估自我更新标志物 (ROS水平,Oct4,Nanog,生长率,差异化).

主要成果:

CTDSPL过度表达导致MSCs的过早衰老,其特征是形态变化,细胞循环停止和衰老标记的增加.
确定miR-18a-5p是CTDSPL的直接调节者,其抑制促进CTDSPL表达和MSC衰老.
过度表达miR-18a-5p减弱了CTDSPL诱导的衰老,降低了ROS水平,增强了自我更新标志物 (Oct4,Nanog),并改善了生长和分化能力.

结论:

在控制MSC衰老方面,miR-18a-5p和CTDSPL之间的动态相互作用至关重要.
在长期培养的MSC中,miR-18a-5p作为一种关键的保护因子,防止长期衰老,保护它们的自我更新和治疗潜力.

关键词:

在CTDSPL中使用CTDSPL.介质细胞干细胞介质细胞干细胞在 miR-18a-5p 中.衰老是一种老化.

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