Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Comparative genomic analysis of clinically relevant human skin-associated fungi.

Nature communications·2026
Same author

Proteomics reveals spatial and molecular heterogeneities in advanced atherosclerotic carotid artery plaques.

Nature cardiovascular research·2026
Same author

Author Correction: Regulatory T cells in the mouse hypothalamus control immune activation and ameliorate metabolic impairments in high-calorie environments.

Nature communications·2026
Same author

Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.

Nature communications·2026
Same author

Deeper is not always better in plasma proteomics.

Nature biotechnology·2026
Same author

AI-Powered Deep Visual Proteomics Reveals Critical Molecular Transitions in Pancreatic Cancer Precursors.

Cancer discovery·2026

相关实验视频

Updated: Jul 1, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

12.8K

阿尔法皮普特 (AlphaPept):一种基于MS的蛋白质组学的现代和开放的框架.

Maximilian T Strauss1,2, Isabell Bludau3, Wen-Feng Zeng3

  • 1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. maximilian.strauss@cpr.ku.dk.

Nature communications
|March 9, 2024
PubMed
概括

AlphaPept是一个新的开源框架,可以加速基于质谱 (MS) 的蛋白质组学数据分析. 这个Python工具显著加快了处理大型数据集的速度,使复杂的蛋白质组分析更有效率.

更多相关视频

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

568
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.1K

相关实验视频

Last Updated: Jul 1, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

12.8K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

568
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.1K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 基于质谱 (MS) 的蛋白质组产生了大量的数据,这带来了重大的分析挑战.
  • 对于和蛋白质的识别和量化,现有的计算工具是众多的,但对于大型数据集来说可能是低效的.

研究的目的:

  • 开发AlphaPept,一个开源的,基于Python的框架,用于高分辨率的MS蛋白质组学数据的高效处理.
  • 利用计算机科学的最新进展,包括即时编译和协作工具,以增强数据分析.

主要方法:

  • 利用Numba在CPU和GPU上进行即时编译,实现了显著的速度改进.
  • 利用Python的科学堆来优化包的性能.
  • 在 Jupyter Notebooks 中实现了识字编程,以促进社区贡献和代码可访问性.

主要成果:

  • 在MS数据处理中实现了百倍的速度改进.
  • 证明了对大型数据集的快速分析,在每个文件的几分钟内处理数百个蛋白质组.
  • 能够创建具有Web服务能力和下游分析兼容性的自动化处理管道.

结论:

  • AlphaPept提供了一个高效的解决方案,用于分析大规模的MS蛋白质组学数据.
  • 该框架的开源性质,模块化设计和易用性鼓励社区参与和进一步开发.
  • AlphaPept显著加速蛋白质组数据分析,使其比数据采集更快.