产前暴露于delta-9-tetrahydrocannabinol会改变 rhesus的胎儿神经发育
Kimberly S Ryan1, Joshua A Karpf2, Chi Ngai Chan3
1Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Mail Code L458, Portland, OR, 97239, USA.
Scientific reports
|March 9, 2024
概括
在中对delta-9-tetrahydrocannabinol (THC) 的产前暴露影响了胎儿的大脑生长和组织学. 分子分析揭示了THC暴露胎儿中的特定微RNA,表明神经发育途径发生了改变.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 产前大麻暴露与后代的不良神经发育结果有关.
- 这些影响背后的精确生物机制在很大程度上是未知的.
- rhesus macaques 作为一个有价值的模型来研究人类胎儿发育.
研究的目的:
- 在 rhesus macaque 模型中研究慢性 delta-9-tetrahydrocannabinol (THC) 暴露对胎儿神经发育的影响.
- 探索与产前THC暴露相关的潜在分子机制,包括microRNA变化.
- 将先进的成像和分子发现与胎儿大脑发育的组织学评估相关联.
主要方法:
- 从怀孕前到怀孕期间,慢性THC给孕妇 rhesus macaques (每组n=5) 的使用.
- 在多个妊娠点 (G85,G110,G135,G155) 进行连续胎儿T2加权MRI,以评估大脑体积发育.
- 产后分析胎儿大脑脊髓液 (CSF) 用于微RNA分析和胎儿大脑组织用于组织学.
主要成果:
- 暴露于THC显示出显著的年龄性相互作用,影响胎儿大脑生长模式.
- 组织学检查显示胎儿大脑组织的异常表明失调.
- 在暴露于THC的胎儿CSF中,确定了两个细胞外囊相关的microRNA,与改变的轴突导向和网林信号通路有关.
结论:
- 在中长期产前THC暴露会诱导胎儿大脑发育的微妙但显著的变化.
- 胎儿CSF中的微RNA失调可能在中介THC的神经发育效应方面发挥作用.
- 需要进一步的研究来确定对后代的长期神经发育后果.
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