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Updated: Jul 1, 2025

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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全长单个蛋白质分子在薄通道中跟踪和计数

Shilo Ohayon1, Liran Taib1, Navneet Chandra Verma1

  • 1Department of Biomedical Engineering, Technion-IIT, Haifa, 3200003, Israel.

Advanced materials (Deerfield Beach, Fla.)
|March 9, 2024
PubMed
概括

这项研究引入了一种新的单分子蛋白质传感方法,用于超快速的无抗体量化. 该技术使完整蛋白质的并行分离和跟踪成为可能,从而推动生物医学研究和诊断.

关键词:
细胞因子面板上的细胞因子.纳米流体的使用蛋白质的异构形式.通过通道进行通道.单颗粒追踪器是一个单颗粒追踪器.单个蛋白质分子的单个蛋白质分子

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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