相关实验视频

Updated: May 2, 2026

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

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使用RumBall RNA-seq分析平台识别差异表达基因的协议.

Luis Augusto Eijy Nagai1, Seohyun Lee1, Ryuichiro Nakato1

  • 1Laboratory of Computational Genomics, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo, Tokyo 113-0032, Japan.

STAR protocols
|March 10, 2024
PubMed
概括

这项研究引入了一种用于RNA测序分析的Dockerized协议,可识别差异表达基因. 该方法确保了从原始数据到解释的基因表达分析的彻底理解.

关键词:
生物信息学是一种生物信息学.在RNA-seqqq.序列分析是指进行序列分析.

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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

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Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq210:10

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Published on: September 18, 2021

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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