相关实验视频

Updated: Jul 31, 2026

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Published on: July 19, 2019

一个 in silico 方案,以优化基于机器学习和虚拟消化自然生物活性的酶获取,基于机器学习和虚拟消化

Like Lin¹, Cong Li¹, Tianlong Zhang¹

¹Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, People's Republic of China.

Analytica chimica acta

|March 10, 2024

概括

一种新的计算方法,SSA-LSTM-VD,改善了自然生物活性 (NBAPs) 的发现. 这种方法准确地预测了活性,并优化了蛋白酶选择,以获得高效的NBAP.

科学领域:

生物技术是生物技术.
生物信息学是一种生物信息学.
酸科学酸科学

背景情况:

自然生物活性 (NBAP) 显示出巨大的潜力,但使用传统方法很难获得.
现有的虚拟消化技术对活性缺乏清晰度,阻碍了高效的NBAP发现.
需要一个高效和准确的策略来获得NBAPs.

研究的目的:

开发一个优化的in silico策略来获取NBAPs.
为了提高自然发现的效率和准确性.

主要方法:

引入了SSA-LSTM-VD,集成了长期短期记忆 (SSA-LSTM) 深度学习与虚拟消化.
SSA-LSTM受过训练,可以从虚拟消化结果中预测活性.
该系统选择最佳的蛋白酶进行实验验证.

主要成果:

SSA-LSTM实现了98.00%的效率,超过了传统的机器学习和基本的LSTM算法.
应用于核桃蛋白质,SSA-LSTM将素确定为最佳蛋白酶 (85.29%的神经保护性).
消化的核桃蛋白 (WPTrH) 在小鼠模型中表现出神经保护作用.

结论:

该SSA-LSTM-VD战略显著提高了NBAP采购的效率和准确性.
这种结合深度学习和虚拟消化方法的方法有助于发现生物活性.
该方法对开发具有神经保护性和其他生物活动的具有前景.

关键词:

生物活性酸是生物活性酸.长期短期记忆长期短期记忆机器学习是机器学习.斯帕罗搜索算法 (Sparrow search algorithm) 是一个非常简单的搜索算法.虚拟消化虚拟消化

更多相关视频

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.