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生物信息学分析和实验验证关键基因与腰椎退化和生物力学相关的基因

Xiyu Liu¹, Lipeng He², Nan Wang¹

¹Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China.

|March 11, 2024

概括

生物力学恶化通过TGF-β通路导致腰椎椎间盘退化 (LDD),涉及SMAD3和TGFB1. 这项研究确定了关键基因和30种潜在的LDD治疗药物.

科学领域:

生物医学工程生物医学工程
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

腰椎间盘退化 (LDD) 是腰部疼痛的主要原因,通常与生物力学压力有关.
现有的LDD研究缺乏生物力学因素和分子机制之间的全面相关性.

研究的目的:

使用生物信息学识别涉及LDD的关键基因,这些基因是由生物机械恶化造成的.
探索LDD的潜在治疗点和候选药物.

主要方法:

利用生物信息数据库 (基因卡,OMIM,药品银行) 来识别与LDD相关的基因.
进行了基因本体学 (GO) 和KEGG通路分析,构建了蛋白质-蛋白质相互作用 (PPI) 网络.
使用GSE56081数据集,RT-PCR和西部斑分析验证了关键基因.

主要成果:

确定了304个与生物力学诱导的LDD相关的基因,主要与炎症和亡有关.
发现了四个枢纽基因:SMAD3,CAV1,SMAD7和TGFB1,其中SMAD3和TGFB1在变性样本中表达高.
证实了TGF-β信号通路在LDD病变发生过程中的参与,并确定了30种潜在药物.

结论:

生物力学恶化驱动LDD通过TGF-β信号通路,其中SMAD3和TGFB1是关键目标.
这项研究为LDD的新型诊断和治疗策略提供了基础.

关键词:

生物信息学是一种生物信息学.生物力学生物力学基因基因基因基因腰椎盘退化腰椎盘退化针对性的药物针对性的药物

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