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Updated: Jul 1, 2025

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem

Published on: December 30, 2015

卷电子显微镜揭示了死后人类前额皮层中的3D突触纳米架构.

Jill R Glausier¹, Cedric Bouchet-Marquis², Matthew Maier¹

¹Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA.

bioRxiv : the preprint server for biology

|March 11, 2024

概括

研究人员在人类大脑组织上使用先进的电子显微镜推断体内突触功能. 这种技术揭示了详细的超结构,为大脑活动和可塑性提供了洞察力.

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Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

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Last Updated: Jul 1, 2025

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem

Published on: December 30, 2015

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

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Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

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Published on: July 9, 2016

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
显微镜的使用方法

背景情况:

突触功能与可以通过电子显微镜 (EM) 观察到的超结构特征相关.
这种相关性表明,可以从ex vivo人类脑组织分析中推断出体内突触功能.

研究的目的:

通过体积电子显微镜 (VEM) 从活体人类大脑组织推断 in vivo 突触功能的可行性.
使用聚焦离子束扫描电子显微镜 (FIB-SEM) 分析人类背侧前额皮层 (DLPFC) 的超结构.

主要方法:

聚焦离子束扫描电子显微镜 (FIB-SEM) 用于高分辨率的三维 (3D) 微图数据集生成.
分析的重点是死后人类背侧前额皮层 (DLPFC) 组织.
测量突触,亚突触和有机细胞的量化.

主要成果:

人类DLPFC的超结构性测量与实验模型一致,不受死前或死后条件的影响.
3D神经重建发现了一个独特的,复杂的棘状树突轴.
这个轴显示了超结构特征,表明了增强的突触通信,集成和可塑性.

结论:

活体VEM分析提供了一种有效的方法,用于推断人脑组织中的活体突触功能.
这项研究证明了使用VEM在超结构水平上研究人类大脑功能的概念证明.
研究结果强调了VEM在神经科学研究中的潜力,以了解人类大脑动态.