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相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

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相关实验视频

Updated: Jul 1, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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从粒子轨迹中学习连续的2D扩散地图,而无需数据结合.

Vishesh Kumar1,2, J Shepard Bryan1,2, Alex Rojewski1,2

  • 1Center for Biological Physics, Arizona State University, USA.

bioRxiv : the preprint server for biology
|March 11, 2024
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种使用高斯过程 (GP) 先验的贝叶斯方法,用于绘制细胞膜的扩散系数. 该方法提供了对膜特性的详细见解,而无需数据结合.

科学领域:

  • 生物物理学的生物物理.
  • 计算生物学 计算生物学

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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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相关实验视频

Last Updated: Jul 1, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
00:10

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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  • 膜动力学 膜动力学

    • 背景情况:

    • 在细胞膜内,扩散系数在空间上有所不同,从而提供了对局部性质 (如组成和刚性) 的见解.
    • 量化这些变化需要超越传统方法的先进分析方法.
    • 单分子成像提供了丰富的数据,但需要复杂的分析扩散映射.

    结论:

    • 这种无监督的贝叶斯式方法准确地绘制了细胞膜上的扩散系数及其不确定性.
    • 该技术为研究局部膜异质性和动态提供了一个强大的新工具.
    • 它通过提供高分辨率的扩散信息,促进了对膜生物物理学的理解.