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相关实验视频

Updated: Jul 1, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

使用纳米孔测序评估初级人体组织的甲基化检测.

Rylee Genner^1,2, Stuart Akeson³, Melissa Meredith⁴

¹Center for Alzheimer's and Related Dementias, National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.

bioRxiv : the preprint server for biology

|March 11, 2024

概括

此摘要是机器生成的。

与R9.9相比,新的纳米孔R10测序化学显示了检测5-甲基素 (5mC) DNA甲基化的高度一致性. 这一进步有助于大规模的测序工作和不同纳米孔化学的数据比较.

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

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Last Updated: Jul 1, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

DNA甲基化,主要是5-甲基 (5mC),在人类基因组中至关重要,与疾病有关.
单分子测序技术 (牛津纳米孔技术和太平洋生物科学) 允许本地DNA分析,包括甲基化检测.
牛津纳米孔技术 (ONT) 发布了R10化学,提高了准确性和吞吐量,但其对5mC检测的影响仍然未被描述.

研究的目的:

在ONT R9和R10纳米孔测序化学之间的5-甲基 (5mC) 检测差异的计算特征.
用不同的人类基因组数据集 (细胞系,大脑,血液) 来比较R9和R10的5mC检测.
评估纳米孔化学对CpG岛屿和同聚合物区域中甲基化检测的影响.

主要方法:

来自纳米孔R9和R10测序数据的5mC调用的计算分析.
在三个人类基因组数据集中比较甲基化检测.
对CpG岛屿和同聚合物区域进行深入分析,以获得甲基化精度.
与Illumina二硫酸盐测序数据的相关性分析.

主要成果:

在所有测试的人类数据集中,在纳米孔R9和R10化学物质之间观察到5mC检测的高一致性.
在细胞系数据中,纳米孔R10与Illumina二硫酸盐测序的相关性最强.
化学品之间的甲基化数据中发现了微妙的差异,这可能会影响下游分析工具,如差异甲基化调用器.

结论:

纳米孔R9和R10甲基化数据之间的比较是可行的指导假设.
这项研究为协调使用不同纳米孔化学物质的大规模测序项目的数据提供了关键的见解.
这些发现支持使用纳米孔R10用于基因组和表观基因组研究中可靠的5mC检测.