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相关概念视频

Tumor Immunotherapy01:27

Tumor Immunotherapy

524
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
524
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

908
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
908
Cancer Vaccines01:30

Cancer Vaccines

367
Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
367
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

1.6K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
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T Cell Types and Functions01:24

T Cell Types and Functions

1.0K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
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相关实验视频

Updated: Jul 1, 2025

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
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PIKfyve控制树突细胞功能和瘤免疫力.

Jae Eun Choi1,2, Yuanyuan Qiao1,2,3, Ilona Kryczek4,5

  • 1Department of Pathology, University of Michigan, Ann Arbor, MI, USA.

bioRxiv : the preprint server for biology
|March 11, 2024
PubMed
概括

向PIKfyve增强树突细胞 (DC) 功能,改善癌症免疫疗法. 抑制PIKfyve增强了DC介导的T细胞免疫力,并增强了免疫检查点阻塞 (ICB) 的有效性,为癌症疫苗提供了新的策略.

关键词:
在 NF-κBB 中.在PIKfyve中使用PIKfyve.抗原特异性T细胞的抗原特异性T细胞.在Apilimod中使用.癌症疫苗 疫苗 癌症疫苗树突细胞的树突细胞.免疫检查点封锁封锁这是一种激酶抑制剂 (kinase inhibitor).瘤是一个瘤.

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相关实验视频

Last Updated: Jul 1, 2025

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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科学领域:

  • 在瘤学瘤学.
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 癌症治疗越来越多地使用免疫疗法和向疗法,如蛋白质激酶抑制剂.
  • 将向癌症的药物与树突细胞 (DC) 调动和免疫疗法的有效性联系在一起的机制尚不清楚.

研究的目的:

  • 研究PIKFYVE基因表达在患者对免疫检查点阻塞 (ICB) 的反应中的作用.
  • 探索PIKfyve抑制对DC功能和抗瘤免疫力的影响.

主要方法:

  • 分析PIKFYVE表达与癌症患者的ICB反应的关系.
  • 在树突细胞中基因切除和药理上抑制PIKfyve.
  • 在临床前小鼠模型中评估瘤生长,T细胞免疫力和ICB疗效.
  • 对阿皮利莫德 (PIKfyve 抑制剂) 与疫苗辅助剂联合使用的评估.

主要成果:

  • 高PIKFYVE表达与较差的ICB完全响应和降低的DC功能相关.
  • 在DC中进行PIKfyve切除,通过非正规NF-κB通路增强了它们的功能.
  • 抑制PIKfyve (遗传或apilimod) 抑制了瘤生长,并改善了DC依赖的T细胞反应.
  • 阿皮利莫德治疗增强了ICB的有效性,并且,与疫苗辅助剂一起,在体内减少了瘤的进展.

结论:

  • PIKfyve作为树突细胞功能的负调节剂.
  • 抑制PIKfyve显示出增强癌症免疫疗法和疫苗策略的巨大潜力.