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通过高通量成像对转录和染色质的基因级可视化

Faisal Almansour¹, Adib Keikhosravi¹, Gianluca Pegoraro¹

¹National Cancer Institute, National Institute of Health.

Research square

|March 11, 2024

概括

高通量光在位杂交 (FISH) 方法检测DNA和RNA在单个基因等位基因中. 这允许精确地绘制单基因水平上的染色质特征和基因活性.

科学领域:

基因组学就是基因组学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

细胞核内的基因组组织对细胞功能至关重要,影响基因表达和调节.
基因组组织表现出显著的单细胞变异性,需要高分辨率的成像技术.
了解等位基因特异性基因组组织是解读基因调节的关键.

结论:

优化的DNA/RNA FISH方法为高通量分析提供了多功能和敏感的工具.
这些方法可以精确地绘制单基因水平上的染色质特征和基因活性.
方便对基因组组织及其对基因调节的影响进行详细的研究.

关键词:

鱼类的DNA/RNA是什么基因组组织基因组组织高通量成像技术的成像在现场混合化.

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相关概念视频

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...