灭菌对释放氧化的葡萄糖传感器的影响
Taron M Bradshaw1, Courtney R Johnson1, Christopher A Broberg1
1Department of Chemistry, University of North Carolina at Chapel Hill, North Carolina, 27599, United States.
概括
通过释放氧化 (NO) 来消毒葡萄糖传感器对于临床使用至关重要. 紫外线 (UV) 光有效消毒NO释放的葡萄糖传感器,而不会影响性能或NO有效载荷.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 传感器技术 传感器技术
背景情况:
- 从S-nitrosothiol修饰的半孔性二氧化纳米颗粒中释放的氧化 (NO) 可以减轻葡萄糖传感器中的异物反应.
- 无释放葡萄糖传感器的临床翻译需要评估绝育方法,因为无捐赠者的敏感性.
研究的目的:
- 评估各种灭菌方法对NO释放葡萄糖传感器的影响.
- 为了确定商业化潜力的最佳灭菌技术.
主要方法:
- 评估的乙烯氧化物,70%的乙醇 (EtOH) 和紫外线 (UV) 光消毒.
- 确定对细菌的10-6无菌保证水平的灭菌周期时间.
- 评估了灭菌成功,没有有效载荷,没有释放持续时间,以及台式传感器性能.
主要成果:
- 乙烯氧化物导致过早的NO释放.
- 70%的EtOH灭菌减少了NO释放的持续时间.
- 通过紫外线灭菌 (大约. 10分钟) 在不损害NO有效载荷或持续时间的情况下实现了无菌性.
- 长期储存 (-20°C和-80°C) 保存了NO分别释放6个月和12个月.
结论:
- 紫外线是NO释放葡萄糖传感器最有前途的灭菌方法.
- 灭菌方法显著影响NO的有效载荷和释放特性.
- 优化灭菌和储存是NO释放葡萄糖传感器临床转换的关键.
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