相关实验视频
Updated: Aug 14, 2026

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Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Published on: July 7, 2014
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简单的拯救之前被iDISCOCO清除的不透明组织
Haylee Mesa1, Jonathan Meade1, Paula Gajewski-Kurdziel2
1Department of Chemistry and Biochemistry, Charles E. Schmidt College of Science, Florida Atlantic University, Boca Raton, FL, USA.
Bio-protocol
|March 11, 2024
概括
以前清除的组织可能会随着时间的推移变得不透明. 这项研究介绍了一种简单的iDISCO-based协议,用于重新清除不透明的样本,保持长期成像的免疫光.
科学领域:
- 神经科学是一个神经科学.
- 生物技术是生物技术.
- 显微镜的使用方法
背景情况:
- 组织清除技术使生物样本的3D可视化成为可能.
- 像iDISCO这样的疏水方法很受欢迎,但清除的组织可能会随着时间的推移变得不透明.
- 这种不透明性阻碍了长期成像和重复分析.
研究的目的:
- 开发一个简单的协议,以拯救不透明的,先前清除的组织样本.
- 为了使免疫标记样本的长期成像.
主要方法:
- 一个经过修改的iDISCO协议,涉及甲醇脱水,二甲脱脂和二乙烯 (DBE) 折射率匹配.
- 该协议应用于先前清除的不透明小鼠大脑.
主要成果:
- 该协议有效地重新清除了不透明的老鼠大脑.
- 在获救的样本中没有观察到可检测的免疫光信号损失.
- 启用了几个月存储的样本的有效重新成像.
结论:
- 这种简单的再覆盖协议有效地挽救不透明的组织样本.
- 它允许扩展免疫标记样本的成像,而不会损害信号完整性.
- 方便纵向研究和重复分析清除的组织.
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