在患有糖尿病外围神经病变的患者中识别和验证与NAD+代谢相关的生物标志物
Chenhao Ye1, Yuedong Fu1, Xijie Zhou1
1Department of Hand and Microsurgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Frontiers in endocrinology
|March 11, 2024
概括
这项研究确定ENPP3和NUDT12是尼古丁胺氨酸二核酸 (NAD+) 代谢相关基因 (NMRGs) 糖尿病外围神经病变 (DPN) 的关键生物标志物. 这些发现为DPN机制和潜在的治疗点提供了新的见解.
科学领域:
- 基因组学和生物信息学
- 代谢途径 代谢途径
- 神经科学是一个神经科学.
背景情况:
- 尼古丁胺氨酸二核酸 (NAD+) 与代谢相关的基因 (NMRGs) 在糖尿病外围神经病变 (DPN) 中的作用仍然在很大程度上是未知的.
- 识别新的生物标志物对于理解和潜在治疗DPN至关重要.
研究的目的:
- 确定可以作为DPN生物标志物的新型NMRG.
- 探索DPN的潜在分子机制和潜在的治疗点.
主要方法:
- 使用基因表达综合 (GEO) 数据集 (GSE95849,GSE185011) 进行差异性基因表达分析.
- 使用八种算法构建了蛋白质与蛋白质相互作用 (PPI) 网络并选了生物标记物.
- 进行了基因组丰富分析 (GSEA) 并构建了调控网络 (lncRNA-miRNA-mRNA,ceRNA).
主要成果:
- 在DPN样本中确定了21种差异表达的NMRG (DE-NMRG).
- ENPP3和NUDT12被验证为DPN的上调生物标志物,显示出诊断疗效.
- GSEA表明ENPP3在Toll-like受体通路中的丰富和NUDT12在糖尿病和胰岛素通路中的丰富.
结论:
- ENPP3和NUDT12被确定为可能参与DPN病变的关键基因.
- 这些发现为进一步研究DPN机制和治疗策略提供了基础.
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