源自人参的I型rhamnogalacturonan多糖化合物与 galectin-8 结合并对抗其功能
Yi Zheng1, Yunlong Si1, Xuejiao Xu1
1Engineering Research Center of Glycoconjugates Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China.
Journal of ginseng research
|March 11, 2024
概括
红 ginseng 果仁素多糖,特别是 RG-I 与 β-1,4- galaktan 侧链,结合和抑制 Galectin-8 (Gal-8). 这项研究澄清了针对Gal-8的潜在药物开发的加勒素-糖相互作用.
科学领域:
- 葡萄糖科学 (Glycoscience) 是一种科学.
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 帕纳克斯人参的多糖化物调节了加勒-3的功能.
- 加勒-8 (Gal-8) 在生物过程和病理学中至关重要.
- 人参多糖对Gal-8的影响在很大程度上是未知的.
研究的目的:
- 为了研究人参衍生的多糖和Galactin-8.8之间的相互作用.
- 为了确定人参培中哪些成分负责Gal-8结合.
- 评估这种相互作用的功能后果.
主要方法:
- 隔离和酶性消化P.人参pectin以产生多糖.
- 生物层干涉测量 (BLI) 用于量化对Gal-8的结合亲和力.
- 测试血液凝结,细胞迁移和T细胞亡,以评估抑制作用.
主要成果:
- 人参培多糖类含有丰富的rhamnogalacturonan-I (RG-I) 和homogalacturonan (HG) 的.
- RG-I,特别是它的β-1,4-银河系侧链,显示出高亲和力结合Gal-8 (亚微分子KD).
- 结合涉及N端和C端的Gal-8域,并与功能抑制相关.
结论:
- P.人参RG-Ipectin的β-1,4-银酸侧链对于结合和对抗Gal-8至关重要.
- 这项研究有助于我们更好地理解加勒素与碳水化合物的相互作用.
- 这些发现可能有助于开发针对Gal-8的新药剂.
相关概念视频
Proteoglycans
3.9K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
3.9K
GPCRs Regulate Adenylyl Cylase Activity
5.5K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.5K
GPCR Desensitization
6.0K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.0K
Glycocalyx and its Functions
4.0K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
4.0K
Glucagon-like Receptor Agonists
321
Incretins include glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), which stimulate insulin secretion post-meals. In type 2 diabetes, GIP's efficacy is reduced, making GLP-1 a viable drug target. GIP originates from preproGIP.
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by...
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by...
321
Glycosaminoglycans
4.8K
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
4.8K


