在无氧和随后的再空气化下,小麦和大米幼苗中的甲酸盐-谷氨酸循环
V V Yemelyanov1, E G Prikaziuk2, V V Lastochkin3
1Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, St. Petersburg, Russia Department of Plant Physiology and Biochemistry, Faculty of Biology, Saint Petersburg State University, St. Petersburg, Russia.
Vavilovskii zhurnal genetiki i selektsii
|March 11, 2024
概括
小麦苗在无氧和再空气过程中经历了氧化应激,而耐药大米保持了抗氧化功能. 这突出了亚酸盐-谷氨循环 (AGC) 对缺氧和恢复反应的差异.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 压力生物学 压力生物学
背景情况:
- 亚酸盐-谷氨循环 (AGC) 对于植物抗氧化防御抗低氧和再空气等压力因素至关重要.
- 了解压力下的AGC酶和成分活性对于作物弹性至关重要.
研究的目的:
- 调查AGC在低氧耐受性大米和低氧敏感小麦中的作用.
- 为了比较AGC酶和基因表达对这两种物种无氧和再气化的反应.
主要方法:
- 在小麦和大米幼苗中对酸和谷氨酸氧化还原状态的比较分析.
- 在芽和根中测定关键的AGC酶活动 (酸盐过氧化酶,脱酸盐减少酶,单一酸盐减少酶,谷氨减少酶).
- 定量PCR (qPCR) 用于分析不同氧气条件下的AGC相关基因的表达.
主要成果:
- 小麦积累了氧化甲酸盐和谷氨,表明氧化应激,而大米保持了减少的形式.
- 在无氧/再气化下,AGC酶活性在小麦中下降,但在大米中保持或增加.
- 无氧和再气化对大米的AGC基因表达进行了差异调节,不同的细胞器和细胞质形式被激活.
结论:
- 小麦的AGC功能因无氧和再通风而受损,导致氧化应激.
- 米表现出强大的AGC活性和基因调节,确保无氧期间和之后的抗氧化能力.
- 与小麦相比,不同的AGC调节有助于米的低氧耐受性.
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