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Updated: Jul 1, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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工程和应用LacI突变体具有严格的表达式

Jieyuan Wu1,2, Chaoning Liang1, Yufei Li1,2

  • 1CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Microbial biotechnology
|March 11, 2024
PubMed
概括

工程 LacI 蛋白 (M7 突变) 显著降低了 lac 促进体的泄漏性,并扩大了诱导范围. 这一突破使得改进的生物传感器和增强的银河系-寡糖 (GOS) 生产.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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