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杜皮卢马布在体外预防了由IL-4引起的鼻上皮功能改变:其有效性的证据

Maxime Fieux^1,2,3,4, Florent Carsuzaa^5,6, Yvan Bellanger^3,4,7

¹Hospices Civils de Lyon, Centre Hospitalier Lyon Sud, Service d'ORL, d'Otoneurochirurgie et de Chirurgie Cervico-Faciale, Pierre Bénite, France.

International forum of allergy & rhinology

|March 11, 2024

概括

杜皮卢马布在体外有效地恢复了鼻上皮的功能,通过抵消互白素-4 (IL-4) 的作用. 这表明dupilumab的存在.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
呼吸系统医学呼吸系统医学

背景情况:

带有鼻息肉的慢性鼻炎 (CRSwNP) 具有2型炎症的特征,包括介质蛋白-4 (IL-4) 和IL-13.
杜皮卢马布向IL-4受体α子单元,抑制IL-4和IL-13信号传递.
皮质功能障碍被假设在CRSwNP的发病过程中发挥作用.

研究的目的:

为了研究IL-4,IL-13,dupilumab在鼻上皮的功能上的体外效应.
确定IL-4和IL-13是否直接影响表皮屏障功能,纤毛运动和伤口愈合.

主要方法:

来自CRSwNP患者的人类鼻上皮细胞 (HNEC) 暴露于IL-4,IL-13和dupilumab.
皮质屏障功能通过跨皮质电阻和紧结蛋白表达来评估.
在48小时刺激后,测量了动和伤口愈合率.

主要成果:

在HNEC中,IL-4显著降低了ocludin和zonula-occludens蛋白质的表达,状脉拍打效率和伤口修复.
IL-13并没有显著影响这些上皮功能.
杜皮卢马布恢复了因IL-4刺激而受损的所有上皮功能.

结论:

在体外,IL-4破坏了鼻上皮质屏障的完整性,状功能和伤口修复.
杜皮卢马布有效地抵消了IL-4对鼻上皮的有害作用.
这些发现支持dupilumab在CRSwNP中通过恢复上皮质完整性来发挥潜在的治疗作用.

关键词:

慢性犀牛鼻炎慢性鼻炎状细胞的移动性皮质细胞上皮质细胞上皮质细胞副鼻腔鼻疾病副鼻疾病

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