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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Stereoisomerism02:52

Stereoisomerism

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Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Stereoisomerism of Cyclic Compounds02:33

Stereoisomerism of Cyclic Compounds

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In this lesson, we delve into the role of ring conformation and its stability, which determines the spatial arrangement and, consequently, the molecular symmetry and stereoisomerism of cyclic compounds. 1,2-Dimethylcyclohexane is used as a case study to evaluate the possible number of stereoisomers. Here, given the multiple (n = 2) chiral centers, there are 2n = 4 possible configurations that lack a plane of symmetry, as the ring skeleton exists in a non-planar chair conformation. In addition,...
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Disubstituted Cyclohexanes: cis-trans Isomerism02:37

Disubstituted Cyclohexanes: cis-trans Isomerism

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Depending upon the different spatial orientation of the substituents, the disubstituted cycloalkanes exhibit two types of stereoisomers. The cis isomers have the substituents on the same side of the ring, whereas the trans isomers have the substituents on the opposite sides. These stereoisomers exhibit different physical properties and cannot be interconverted without breaking the carbon-carbon bonds.
In cyclohexane, the substituents can occupy different positions generating distinct isomers....
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Selectins01:25

Selectins

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Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
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相关实验视频

Updated: Jul 1, 2025

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
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在血清中,CL-11以功能上不同的异构体的形式循环.

Adrian Sutta1,2, Nelia Nina Leemans1, Michael Ploug3,4

  • 1Laboratory of Molecular Medicine, Department of Clinical Immunology, Copenhagen University Hospital: Rigshospital, Copenhagen, Denmark.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology
|March 11, 2024
PubMed
概括

集合素-11 (CL-11) 异型A和D表现出不同的生物功能. CL-11D与集蛋白-10 (CL-10) 和较低的连接体结合的相互作用减少,这可能会限制其补充激活作用.

关键词:
在 CL-10 中,我们可以看到 CL-10 .在CL-11中,我们可以看到在MASP MASP上收集并收集这些信息.互补系统是互补的系统.莱克通路的路径

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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 补充系统 补充系统

背景情况:

  • 集合素-11 (CL-11) 是一个在莱克补充通路中的模式识别分子.
  • 替代性拼接COLEC11基因产生血清异型A和D,不同于原样区域的长度.
  • 这些异构体的生物学差异和对补体激活的影响尚未完全理解.

研究的目的:

  • 阐明CL-11异型A和D之间的生物学差异.
  • 调查它们与集合素-10 (CL-10) 和MASPs的相互作用.
  • 评估它们在补充级联激活中的潜在作用.

主要方法:

  • 生产复合CL-11异型 (A和D) 和CL-10/11复合体.
  • 对CL-10/11异构复合物的形成和稳定性的分析.
  • 血清CL-11的免疫沉,然后进行质谱学.
  • 评估CL-11异型体的MASP和配体结合能力.

主要成果:

  • 两种CL-11异型与CL-10相关,其中CL-11D的结合性降低.
  • 形成了CL-10/11异构复合物,表现出比同类三元体更大的稳定性.
  • 原生CL-11以CL-10/11与MASP-1和MASP-3的异构复合体的形式循环.
  • 与CL-11A相比,CL-11D结合的MASP,但与CL-11A相比,结合带的结合减少.
  • 在CL-11D中显示了减少的寡合化和异构复合体的形成.

结论:

  • CL-11D的较短的原区域不会阻止MASP结合,但会减少连接体相互作用和异构复合体的形成.
  • 与全长CL-11A异型相比,CL-11D可能具有有限的补体激活潜力.
  • 这些发现突出了CL-11异型体在补充调节中的功能区别.