土壤微生物组及其在长期高污染土壤中的功能基因
Prinpida Sonthiphand1, Nattanan Rueangmongkolrat2, Pichahpuk Uthaipaisanwong3
1Department of Biology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand. prinpida.son@mahidol.ac.th.
Bulletin of environmental contamination and toxicology
|March 11, 2024
概括
由arsM基因驱动的微生物甲基化是缓解高度污染的土壤中毒性的关键. 这一过程影响了土壤微生物群.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学生物地质化学
- 土壤科学 土壤科学
背景情况:
- 微生物的转化对于管理污染和降低相关风险至关重要.
- 土壤中高污染给环境带来了重大挑战.
研究的目的:
- 研究高污染下土壤微生物组的时间和空间动态.
- 为了确定在梯度沿线的功能基因的多样性和丰富性.
主要方法:
- 结合高通量测序和基于PCR的方法,针对16SrRNA和的功能基因.
- 炮弹元基因组学用于识别一系列功能基因 (arsM,arsC,arrA,aioA).
主要成果:
- 土壤微生物组主要由阿克提诺细菌,蛋白质细菌,菌菌 (Bacillus),流体和酸性细菌主导.
- 只有arsM基因通过PCR检测到,相对丰度为0.20%-1.57%.
- 由arsM基因介导的甲基化被确定为一个关键过程.
结论:
- 通过arsM基因甲基化是生物地球化学循环中的关键机制.
- 这种微生物过程在减轻受污染的土壤中的毒性方面发挥着重要作用.
- 这项研究增强了对长期,高度污染的土壤中的生物地质化学的理解.
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