IFI16积极调节RIG-I介导的I型干扰素生产,以一种独立于STING的方式
Xibao Shi1, Menglu Wei1, Yuwen Feng1
1College of Life Sciences, Henan Normal University, Xinxiang, China.
DNA and cell biology
|March 11, 2024
概括
干扰素马诱导蛋白16 (IFI16) 增强RNA传感器RIG-I信号,用于独立于STING的I型干扰素生产. 这一发现澄清了IFI16.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 干扰素马诱导蛋白16 (IFI16) 作为DNA和RNA传感器,对I型干扰素 (IFN-I) 诱导至关重要.
- 已经证明IFI16与cGAS协同作用,并增强IFN-I生产的RIG-I/MAVS通路.
- 在IFI16介导的RNA传感途径中,STING的确切作用尚不清楚.
研究的目的:
- 调查IFI16是否以STING-依赖的方式调节RNA传感器RIG-I-MAVS-IFN-I通路.
- 阐明IFI16影响对RNA病毒的先天免疫反应的机制.
主要方法:
- 利用缺乏内源性STING的HEK 293T细胞来评估先天免疫反应.
- 用DNA转染的细胞来评估STING独立的信号传递.
- 在IFI16的存在下,通过RIG-I和MAVS调解的IFN-I诱导测量.
主要成果:
- IFI16增强了RIG-I和MAVS介导的I型干扰素 (IFN-I) 诱导,以独立于STING的方式.
- 该机制不涉及IFI16.16对NF-kappa-B基本调节器 (NEMO) 的上调.
- IFI16在RNA传感途径中的作用独立于STING-cGAMP-TBK1轴.
结论:
- IFI16调节RNA-RIG-I-MAVS-IFN-I信号通路,独立于STING的作用.
- 这一发现突显了IFI16在抗病毒先天免疫中的STING独立机制.
- 需要进一步的研究,以完全界定IFI16在先天免疫传感中的功能.
更多相关视频
相关概念视频
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K


