旗菌ST34类型的表型变异沙门氏菌 Typhimurium和变种
Xin Wu1, Fanan Suksawat2, Patchara Phuektes2
1Yunnan Joint International R&D Center of Veterinary Public Health, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
Foodborne pathogens and disease
|March 11, 2024
概括
测定沙门氏菌Typhimurium变种的血清型是具有挑战性的. 使用FTIR和wgMLST进行的基因分析显示,ST34菌株中抗原配方和家族遗传关系之间没有相关性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 分子类型的定型.
背景情况:
- 沙门氏菌 (Salmonella enterica serovar Typhimurium) 和其变种是人类沙门氏菌病的主要原因.
- 对于沙门氏菌Typhimurium变异的传统血型鉴定方法在准确识别方面存在重大挑战.
- 之前的研究表明,在Salmonella Typhimurium中的抗原配方和遗传特征之间存在潜在的不一致性.
研究的目的:
- 评估传统的抗原配方与高分辨率的基因定型方法之间的一致性,以检测沙门氏菌Typhimurium变种.
- 通过福里埃变换红外光谱学 (FTIR) 和全基因组多位序列类型化 (wgMLST) 来研究沙门氏菌Typhimurium菌株之间的遗传学关系.
- 评估与抗原配方相关的鞭 (H抗原) 变异的遗传基础.
主要方法:
- 使用脉冲场凝电泳 (PFGE) 进行初始菌株分组.
- 雇佣的富里埃变换红外光谱仪 (FTIR) 用于分子指纹.
- 在14个局部菌株和50个公共ST34沙门氏菌Typhimurium数据上进行了全基因组多部位序列类型化 (wgMLST).
- 分析了来自14个菌株和293个公共ST34菌株的全基因组数据的鞭毛编码基因.
主要成果:
- 脉冲场凝电泳 (PFGE) 显示,抗原配方与基因型之间没有明确的相关性.
- 富里埃变换红外光谱 (FTIR) 和全基因组多位序列类型 (wgMLST) 表明抗原公式和基因型/表型之间缺乏规律性.
- 对58个鞭毛编码基因的分析显示了13种模式,但相同的基因模式可能与不同的抗原配方相对应.
结论:
- 抗原配方与ST34沙门氏菌Typhimurium和变种菌株的真实家族遗传关系之间存在重大不一致.
- 像FTIR和wgMLST这样的高分辨率类型方法与这些菌株的传统血清类型分类不一致.
- 即使是鞭抗原和基因配置文件也不能始终反映沙门氏菌Typhimurium这个群体内的遗传关系,这突显了当前类型化策略的局限性.
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