在聚合物材料中的无细胞蛋白表达
Marilyn S Lee1, Jennifer A Lee1,2, John R Biondo1,3
1U.S. Army Combat Capabilities Development Command Chemical Biological Center, 5183 Blackhawk Road, Aberdeen Proving Ground, Maryland 21010, United States.
ACS synthetic biology
|March 11, 2024
概括
这项研究表明,无细胞蛋白合成 (CFPS) 反应在干燥时可以在苛刻的塑料制造工艺中存活下来. 这使得能够创建具有内置生物功能的智能塑料,例如生产抗微生物药物或感知环境变化.
科学领域:
- 合成生物学 合成生物学
- 材料科学是一种材料科学.
- 生物技术是生物技术.
背景情况:
- 合成生物学的应用往往局限于水性环境.
- 将生物功能纳入塑料等合成材料是具有挑战性的,因为加工条件恶劣.
- 无细胞蛋白合成 (CFPS) 通过在活细胞之外重建细胞功能提供了一个潜在的解决方案.
研究的目的:
- 在聚合物材料加工的背景下,研究干细胞无蛋白合成 (CFPS) 反应的稳定性和实用性.
- 为了证明使用集成到塑料中的CFPS创建功能性材料.
主要方法:
- 干燥的CFPS反应受到高温和有机溶剂暴露,这是聚合物造工艺的典型特征.
- 评估了CFPS组件在暴露后的生存能力和功能.
- 通过结合这些强大的CFPS系统来制造功能性塑料.
主要成果:
- 干燥的CFPS反应在材料造过程中对热量和有机溶剂具有显著的耐受性.
- 该研究成功地创造了具有空间模式遗传功能的塑料.
- 这些工程塑料在现场展示了抗菌剂的生产和传感能力.
结论:
- 无细胞蛋白质合成为将可编程生物功能集成到固态材料中提供了一种可行的方法.
- 这种方法克服了与合成材料中的细胞活力和遗传制相关的局限性.
- 开发的技术为为各种应用创造先进的生物功能塑料开辟了新的途径.
相关概念视频
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Conjugated Proteins
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Conjugated Proteins
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...


