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通过原沉积来调节依赖度的巨细胞炎症反应

Vijaykumar S Meli^1,2,3, Andrew T Rowley³, Praveen K Veerasubramanian^1,2

¹Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697, United States.

ACS biomaterials science & engineering

|March 11, 2024

概括

基质功能化影响巨细胞的炎症反应. 溶剂系统的原附着显著改变免疫细胞的行为和机械感知,无论水凝的刚性.

科学领域:

免疫生物学的免疫生物学
生物材料科学生物材料科学
细胞机械感知细胞机械感知

背景情况:

巨细胞是与生俱来的免疫细胞,与细胞外基质相互作用.
原蛋白在免疫细胞培养中被广泛使用,但基质功能化往往被忽视.
像矩阵刚度这样的环境线索调节巨细胞活动.

研究的目的:

研究原溶解溶剂如何影响巨细胞炎症激活和机械感知.
确定基质组织在调节免疫细胞反应中的作用.
了解生物材料表面化学对巨细胞行为的影响.

主要方法:

在酸 (Col-AA) 或HEPES (Col-HEP) 中溶解原蛋白,并与聚烯胺水凝结合.
在不同硬度的功能化水凝上培养骨髓衍生的巨细胞.
分析炎症性细胞因子/基因表达,原受体 (LAIR-1) 表达和机械敏感分子局部化 (YAP/TAZ).

主要成果:

不管硬度如何,Col-AA表面诱导了高炎性细胞因子和基因表达.
科尔-HEP表面显示了硬度依赖的炎症激活.
与Col-HEP相比,Col-AA上的巨细胞表现出增强的扩散,素表达和YAP/TAZ核定位.

结论:

对于原沉积的溶剂选择极大地影响了巨细胞的炎症激活和机械感知.
生物材料表面功能化的细节对于可复制免疫生物学研究至关重要.
了解这些影响对于设计有效的免疫调节生物材料至关重要.

关键词:

第一个层是LAIR-1.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈原蛋白是一种原蛋白.免疫细胞是免疫细胞.这是一种炎症炎症炎症炎症.基板的硬度基板的硬度

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