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Updated: Jul 1, 2025

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核酸环境对Bst exo-DNA聚合酶对DNA非特异放大的影响

Ravil R Garafutdinov1, Olga Yu Kupova2, Assol R Sakhabutdinova2

  • 1Institute of Biochemistry and Genetics, Ufa Federal Research Center, Russian Academy of Sciences, Ufa, 450054, Russia. garafutdinovr@gmail.com.

Biochemistry. Biokhimiia
|March 11, 2024
PubMed
概括

这项研究揭示了特定的DNA序列,特别是那些富含3'-末端dG的精氨酸的序列,在使用Bst exo-DNA聚合酶的异热放大过程中促进了不必要的多元化. 避免终端dA和dG核酸可以防止这种问题,以获得可靠的放大.

关键词:
Bst DNA 聚合酶是一种异热放大放大 异热放大放大分子对接的分子对接.多元化的多元化.特殊性的特异性

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