相关实验视频

Updated: Jul 1, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

N⁶-甲基氨酸修饰不是病毒RNA基因组的一般性特征

Belinda Baquero-Pérez¹, Ivaylo D Yonchev^2,3, Anna Delgado-Tejedor⁴

¹Molecular Virology Group, Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Dr. Aiguader 88, 08003, Barcelona, Spain.

Nature communications

|March 12, 2024

概括

这项研究没有发现N6-甲基氨酸 (m6A) 修饰在奇孔古尼亚 (CHIKV) 和登革热 (DENV) 病毒RNA中的证据. 耗尽宿主m6A机械部件并没有影响病毒感染,这挑战了现有的观念.

科学领域:

病毒学病毒学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

在调节RNA功能的过程中,N6-甲基氨酸 (m6A) 表转录体标记至关重要.
虽然m6A机器是核的,但在CHIKV和DENV等细胞质RNA病毒中报告了m6A修饰.
以前的研究结果主要依赖于m6A-Seq,这是一种依赖于抗体的方法,容易产生错误阳性.

研究的目的:

严格调查m6A修饰在奇孔古尼亚病毒 (CHIKV) 和登革热病毒 (DENV) RNA中的存在.
为了验证或驳斥在单独细胞质RNA病毒中广泛存在的m6A修饰.
评估宿主m6A机器在CHIKV和DENV感染中的功能相关性.

主要方法:

结合m6A-Seq与抗体独立技术,如SELECT和纳米孔直接RNA测序.
进行了功能,分子和突变发生的研究.
评估了耗尽宿主m6A机械元件对病毒感染和局部化的影响.

主要成果:

在使用正交验证方法的CHIKV或DENV转录中没有发现m6A修饰的证据.
关键宿主m6A机械部件的耗尽没有影响CHIKV或DENV感染水平.
感染CHIKV和DENV并没有改变宿主m6A机械的局部.

结论:

在细胞质RNA病毒中普遍存在m6A修饰的普遍概念受到挑战.
坐标验证对于确认RNA修改至关重要,尤其是在使用抗体依赖方法时.
m6A修饰不是CHIKV和DENVRNA的一般特征,并且宿主m6A机器对于它们的复制并不重要.

更多相关视频

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...