利波亚比诺曼南在真菌细菌的分裂过程中调解局部细胞壁完整性
Ian L Sparks1, Takehiro Kado1, Malavika Prithviraj1
1Department of Microbiology, University of Massachusetts, Amherst, MA, USA.
Nature communications
|March 12, 2024
概括
甲比诺南 (LAM) 对于菌根细胞的形状和分裂至关重要. 缺乏成熟LAM的突变物在新极点失去棒形状,而改变的LAM则导致多个,突出显示了LAM在细胞外完整性中的作用.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 细菌病原体的产生
背景情况:
- 菌根菌表现出独特的生长和分裂模式,与典型细菌不同.
- 细胞外,特别是像利波曼南 (LM) 和 lipoarabinomannan (LAM) 这样的脂质糖,对于菌根菌的结构和宿主相互作用至关重要.
- 在真菌菌细胞外维护和分裂中,LM和LAM的精确生理作用基本上是未知的.
研究的目的:
- 调查脂质甘油的生理功能,特别是脂 (LM) 和脂 (LAM),在维持细胞外完整性方面.
- 阐明LAM在细胞形状,分裂和菌根菌中隔膜位置方面的作用.
主要方法:
- 在Mycobacterium smegmatis和Mycobacterium tuberculosis中产生和分析生物合成突变的脂糖 (LM和LAM).
- 在野生型和突变菌株中对细胞形状和隔离模式的显微镜检查.
- 细胞壁完整性的评估与脂质甘的生产有关.
主要成果:
- 缺乏成熟的利波亚比诺曼南 (LAM) 生产的突变体在维持杆细胞形状方面表现出缺陷,特别是在新极和 septal 区域.
- 一个产生扩大LAM的突变体显示出异常的多分离,表明分裂过程发生了变化.
- 这些发现表明,LAM对于局部细胞壁完整性和真菌细菌分裂期间正确的隔膜定位至关重要.
结论:
- 甲比诺南 (LAM) 在菌根细菌细胞分裂中起着关键的,位置特定的作用.
- 拉姆对于在新极端和对 septal 区域保持细胞壁完整性至关重要.
- 拉姆影响了七的放置和形成,影响了菌根菌中的整体细胞形状和分裂忠实度.
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